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recombinant psgl 1  (Sino Biological)


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    Structured Review

    Sino Biological recombinant psgl 1
    Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 <t>(PSGL-1)</t> residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.
    Recombinant Psgl 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+psgl+1/Human+PSGL-1+%2F+CD162+%2F+SELPLG+Protein/pmc12969431-287-1-3
    Average 94 stars, based on 7 article reviews
    recombinant psgl 1 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "The protease cathepsin K can debulk the cancer glycocalyx"

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111206

    Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 (PSGL-1) residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.
    Figure Legend Snippet: Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 (PSGL-1) residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.

    Techniques Used: Generated, Mass Spectrometry, Modification, Purification, Recombinant, Glycoproteomics, Residue

    Related Articles

    Purification:

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx
    Article Snippet: .. Purified recombinant PSGL-1 (Sino Biological; 13863-H08H) was labeled with IRDye 800CW NHS Ester (LI-COR Biosciences) following the manufacturer’s recommendations. .. IR800 recombinant PSGL-1 and IR800 purified MUC16 were deglycosylated using protein deglycosylation mix II (New England Biolabs).

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx
    Article Snippet: .. Recombinant PSGL-1 (10 μg; Sino Biological; 13863-H08H), recombinant MUC1 (Sino Biological; 12123-H05H), recombinant CD43 (R&D Systems; 9680-CD-050), fetuin (Promega; V4961), and purified MUC16 (purified from OVCAR-3 as above) were mixed with CTSK or PBS control at a 1:50 CTSK:substrate molar ratio in 1:1 PBS:100 mM sodium acetate with a final pH of 5 (buffer pHed as above). ..

    Article Title: The protease Cathepsin K can debulk the cancer glycocalyx.
    Article Snippet: .. O-glycoproteomics and cleavage specificity 10 μg of recombinant PSGL-1 (SinoBiological; 13863-H08H), recombinant MUC1 (SinoBiological; 12123-H05H), recombinant CD43 (R&D Systems; 9680-CD-050), fetuin (Promega; V4961), purified MUC16 (purified from OVCAR-3 as above) were mixed with CTSK or PBS control at a 1:50CTSK:substrate molar ratio in 1:1 PBS:100 mM sodium acetate with a final pH of 5 (buffer pHed as above). ..

    Recombinant:

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx
    Article Snippet: .. Purified recombinant PSGL-1 (Sino Biological; 13863-H08H) was labeled with IRDye 800CW NHS Ester (LI-COR Biosciences) following the manufacturer’s recommendations. .. IR800 recombinant PSGL-1 and IR800 purified MUC16 were deglycosylated using protein deglycosylation mix II (New England Biolabs).

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx
    Article Snippet: .. Recombinant PSGL-1 (10 μg; Sino Biological; 13863-H08H), recombinant MUC1 (Sino Biological; 12123-H05H), recombinant CD43 (R&D Systems; 9680-CD-050), fetuin (Promega; V4961), and purified MUC16 (purified from OVCAR-3 as above) were mixed with CTSK or PBS control at a 1:50 CTSK:substrate molar ratio in 1:1 PBS:100 mM sodium acetate with a final pH of 5 (buffer pHed as above). ..

    Article Title: The protease Cathepsin K can debulk the cancer glycocalyx.
    Article Snippet: Gels were imaged using an Odyssey CLx near-infrared fluorescence imaging system (LI-COR Biosciences). .. Recombinant mucin in vitro digestions 1 μg of recombinant PSGL-1 (SinoBiological; 13863-H08H) or MUC1 (SinoBiological; 12123- H05H) were incubated with each cathepsin at a 1:50 enzyme:susbtrate molar ratio for 16 hours in 37 °C in 1:1 PBS:100 nM sodium acetate witha final pH of 5, 6, or 7 (buffer pHed as above). .. Samples were subject to SDS-PAGE using 4–12% Criterion XT Bis-Tris protein gel (Bio-Rad) and run in XT-MOPS (Bio-Rad) at 180 V for 1 hour.

    Article Title: The protease Cathepsin K can debulk the cancer glycocalyx.
    Article Snippet: .. O-glycoproteomics and cleavage specificity 10 μg of recombinant PSGL-1 (SinoBiological; 13863-H08H), recombinant MUC1 (SinoBiological; 12123-H05H), recombinant CD43 (R&D Systems; 9680-CD-050), fetuin (Promega; V4961), purified MUC16 (purified from OVCAR-3 as above) were mixed with CTSK or PBS control at a 1:50CTSK:substrate molar ratio in 1:1 PBS:100 mM sodium acetate with a final pH of 5 (buffer pHed as above). ..

    Labeling:

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx
    Article Snippet: .. Purified recombinant PSGL-1 (Sino Biological; 13863-H08H) was labeled with IRDye 800CW NHS Ester (LI-COR Biosciences) following the manufacturer’s recommendations. .. IR800 recombinant PSGL-1 and IR800 purified MUC16 were deglycosylated using protein deglycosylation mix II (New England Biolabs).

    Control:

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx
    Article Snippet: .. Recombinant PSGL-1 (10 μg; Sino Biological; 13863-H08H), recombinant MUC1 (Sino Biological; 12123-H05H), recombinant CD43 (R&D Systems; 9680-CD-050), fetuin (Promega; V4961), and purified MUC16 (purified from OVCAR-3 as above) were mixed with CTSK or PBS control at a 1:50 CTSK:substrate molar ratio in 1:1 PBS:100 mM sodium acetate with a final pH of 5 (buffer pHed as above). ..

    Article Title: The protease Cathepsin K can debulk the cancer glycocalyx.
    Article Snippet: .. O-glycoproteomics and cleavage specificity 10 μg of recombinant PSGL-1 (SinoBiological; 13863-H08H), recombinant MUC1 (SinoBiological; 12123-H05H), recombinant CD43 (R&D Systems; 9680-CD-050), fetuin (Promega; V4961), purified MUC16 (purified from OVCAR-3 as above) were mixed with CTSK or PBS control at a 1:50CTSK:substrate molar ratio in 1:1 PBS:100 mM sodium acetate with a final pH of 5 (buffer pHed as above). ..

    In Vitro:

    Article Title: The protease Cathepsin K can debulk the cancer glycocalyx.
    Article Snippet: Gels were imaged using an Odyssey CLx near-infrared fluorescence imaging system (LI-COR Biosciences). .. Recombinant mucin in vitro digestions 1 μg of recombinant PSGL-1 (SinoBiological; 13863-H08H) or MUC1 (SinoBiological; 12123- H05H) were incubated with each cathepsin at a 1:50 enzyme:susbtrate molar ratio for 16 hours in 37 °C in 1:1 PBS:100 nM sodium acetate witha final pH of 5, 6, or 7 (buffer pHed as above). .. Samples were subject to SDS-PAGE using 4–12% Criterion XT Bis-Tris protein gel (Bio-Rad) and run in XT-MOPS (Bio-Rad) at 180 V for 1 hour.

    Incubation:

    Article Title: The protease Cathepsin K can debulk the cancer glycocalyx.
    Article Snippet: Gels were imaged using an Odyssey CLx near-infrared fluorescence imaging system (LI-COR Biosciences). .. Recombinant mucin in vitro digestions 1 μg of recombinant PSGL-1 (SinoBiological; 13863-H08H) or MUC1 (SinoBiological; 12123- H05H) were incubated with each cathepsin at a 1:50 enzyme:susbtrate molar ratio for 16 hours in 37 °C in 1:1 PBS:100 nM sodium acetate witha final pH of 5, 6, or 7 (buffer pHed as above). .. Samples were subject to SDS-PAGE using 4–12% Criterion XT Bis-Tris protein gel (Bio-Rad) and run in XT-MOPS (Bio-Rad) at 180 V for 1 hour.



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    Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 <t>(PSGL-1)</t> residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.
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    Image Search Results


    Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 (PSGL-1) residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.

    Journal: The Journal of Biological Chemistry

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx

    doi: 10.1016/j.jbc.2026.111206

    Figure Lengend Snippet: Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 (PSGL-1) residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.

    Article Snippet: Purified recombinant PSGL-1 (Sino Biological; 13863-H08H) was labeled with IRDye 800CW NHS Ester (LI-COR Biosciences) following the manufacturer’s recommendations.

    Techniques: Generated, Mass Spectrometry, Modification, Purification, Recombinant, Glycoproteomics, Residue